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Image Search Results
Journal: Frontiers in Oncology
Article Title: Characterization of Anti-Cancer Activities of Violacein: Actions on Tumor Cells and the Tumor Microenvironment
doi: 10.3389/fonc.2022.872223
Figure Lengend Snippet: Macrophage activation by violacein-killed tumor cell supernatant. HCT116, Huh7, and PANC-1 cells were treated with 50 µM violacein or oxaliplatin for 4 h before the media was changed. Then, 24 h after treatment, supernatants from untreated (co) and compound-treated cells were collected, and dead cells were removed. (A-C) RAW-Blue™, (D-F) THP1-XBlue™, (G, H) HEK-Blue™ hTLR4, and (J-L) HEK-Blue™ Null2 reporter cells were incubated with the respective dead tumor cell-conditioned medium (dTCM, 50% v/v) or LPS (100 ng/ml for RAW-Blue™ and 10 ng/ml for THP1X-Blue™, HEK-Blue™ hTLR4, and HEK-Blue™ Null2) for 24 h. The activation of the reporter cells was determined by colorimetric QUANTI-Blue™.
Article Snippet:
Techniques: Activation Assay, Incubation